MC-1 Unit-2 (Drug Acting on Autonomic Nervous System)

 

   



Introduction:

  •  Autonomic nervous system is a part of nervous system that controls and regulates the internal organs/body.
  • It control involuntary response , e.g. Breathing, digestion.
  • Two type:-

1.     Sympathetic :  >When our body is in abnormal situation  it works(fight or flight situation).                                   >Work such as increase heart rate, decrease digestion rate.

2.     Parasympathetic:> When our body come back from sympathetic nervous system to normal condition.                                                                                                                                    >Works such as normal heart rate & digetion rate

 Adrenergic Neurotransmitters:

     Adrenergic: 

                         Adrenergic drugs are the agents which acts directly on the sympathetic nervous system and these drugs are also known as sympathomimetic drugs as these agents mimics(copy) the actions of sympathetic nervous system.

 

   Neurotransmitters:

                                  Neuro: Neuron ;

                                 transmitter: transfer the information 

                                 Neurotransmitter are chemical messenger that transmit signals from a neuron to a target cell/neurons across a synapse.

  • Sympathetic is called Adrenergic.
  • Parasympathetic is called Cholinergic.
  • Both Adrenergic and Cholinergic division work antagonistically.

                         

  • Dopamine: it is a naturally occurring catecholamine                                                                                       it acts as a neurotransmitter in the basal ganglia of CNS.
  • Epinephrine: It produce in stressful situation, increase heart rate & blood flow.                                          Leading to physical boost & heightened awareness.                                                                It is also named as Adrenaline.                                                                                                               
  • Norepinephrine: It affect attention and responding action in the brain.                                                               Contract blood vessels, increasing blood flow.

 

Biosynthesis and catabolism of catecholamine 

   Catecholamines:    It is a mono amino neurotransmitter, in which catechol ring is present with a side chain amine 

 

Biosynthesis of catecholamine:

                                     The biosynthesis take place in adrenergic and dopaminergic neurons in the CNS, in sympathetic neurons in the ANS and in the adrenal medulla.

 

 

1.     Phenylalanine get hydrolyzed into tyrosine in liver.

2.     Tyrosine get hydrolyzed into DOPA by the enzyme tyrosine hydroxylase, in cytoplasm.

3.     DOPA converted into  Dopamine with the help of enzyme DOPA decarboxylase in which COremoved, in cytoplasm of neurons, forms Dopamine(first catecholamine formed).

4.      Dopamine is converted into nor-adrenaline with the help of enzyme dopamine Î²-hydroxylase, nor-epinephrine formed.

5.     Nor-adrenaline get converted into adrenaline with the help of enzyme Pheylethanolamine-N methyl transferase (PNMT), in the adrenaline medulla.

Catabolism of catecholamine 

                        > It is the removal of Catecholamines and also termination of action of catecholamine. 

                        > In this process, structure of catecholamine changes with the help of enzymes (MAO,COMT) so they do not interact with adrenergic receptor to produce effects. 

 

 Enzymes: 

1.     MAO: Monoamine oxidase(deamination)

2.     COMT: Catechol o-Methyl transferase(methylation in ring)

 

 

Adrenergic receptors and their distribution :

Adrenergic receptor:

                  Adrenergic receptor are those receptor in which adrenergic drugs/neurotransmitter will bind directly to increase various action/response.

  • Adrenergic receptor are membrane bound G-protein coupled receptors.
  • these are classified into two types:

                   > Î± receptors: -α1, Î±2

                   > Î² receptors :- Î²1, Î²2, Î²3

 

 Distribution of adrenergic receptor with their action:

1.      Î±1 receptors: > Present on post synaptic receptor sites.                                                                                            > Smooth muscles of blood vessels (vasoconstriction).                                                                                         Function:  > Gland cells (Gland secretion).                                                                                                                     > Glycogenolysis in liver (glucose synthesis).                                                                                                                                               > also present in iris, bladder, uterus etc.                                                                                                                    -these are mainly excitatory in nature.

2.     Î±2 receptors :Present on pre and post-synaptic receptor sites.                                                                              > Presents  in CNS, Pancreas                                                                                                       Function:> inhibit neurotransmitter release.                                                                                                                    > Decrease insulin release.                                                                                                                                                                           > Platelet aggregation.                                                                                                                                - these are mainly inhibitory in nature. 

3.     Î²1 receptors :Present in cardiac tissue (myocardium) i.e. Heart and in Kidney.                                    Function:> contraction and heart rate increases.                                                                                                                   > Release Renin from Kidney results increase B.P.                                                                                                                                                     - Excitatory in nature.

4.     Î²2 receptors :Present in smooth muscles and gland cells, i.e. Bronchi, Blood vessels, Veins, Liver, G.I.T.                                                                                                                                                     Function:> Relaxation- Bronchodilator, Vasodilatation, Vein relaxation.                                                                          -inhibitory in nature.

5.     Î²3 receptors : >Present in adipose tissue and urinary bladder.                                                                      Function:>Lipolysis, relaxation. 

 

Sympathomimetic Agents: SAR of Sympathomimetic agents:

 

      Sympathomimetic agents:

                            -Also known as adrenergic agonist.

                            -Sympathomimetic - Sympatho- sympathetic nervous system

                                                              mimetic- mimic (copy)

  • Sympathomimetic agent are those chemical agents/drugs which copy the action of sympathetic nervous system. 
  • These agents/drugs bind with adrenergic receptor and give action.(act as adrenergic neurotransmitter.

     SAR of Sympathomimetic agents:

                   SAR:-Structure activity relationship

  • It is defined as the relationship between the chemical structure of a molecules and its  biological activity.
  • This allows modification of the effect or potency of a substance b changing its chemical structure.

     Sympathomimetic agents:-

 

 

    Structurally, Substitution is possible on :-

  • Catechol(aromatic ring)
  • β-carbon
  • α-carbon
  • Amino group
                   Substitution on Aromatic ring:- 

 

  • Presence of -OH group at alternate (3,4 position) gives maximum Î±,β activity, Absence  of these group  decrease Î±,β activity(also their potency)   
  •   But unsubstituted(without -oh group) aromatic ring, easily cross blood brain barrier (BBB) and have more CNS
              Substitution on Î²-carbon:

  -OH group attachment on carbon decrease CNS activity due to lower to lower liquid solubility

 Of the drug .But increase the Î± activity

             e.g.:- Ephedrine


       Substitution on Î±-carbon:

                       Drugs having substitution on carbon block the metabolism (activity of MAO) larger duration of action  

      e.g.:- Amphetamine

         Substitution on amino group:

                            The amino group is essential (primary or secondary), but tertiary are poor direct agonists

         1° and 2° amino group-high Î± selectivity (activity) e.g.:- adrenaline

More the size of alkyl substituent, higher will be Î² selective action and lower Î± selectivity.

 e.g.:- Isoprenaline, salbutamol.

 

 

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